Leica SP8 FALCON
Leica TCS SP8 Upright Confocal Microscope with FALCON System for FLIM, FRET and FCS
Lasers:
Diode Laser 405 nm
Diode Laser, 440 nm (pulsed, up to 40kHz)
Ar Laser 458 nm, 476 nm, 488 nm, 496 nm, 514 nm
DPSS Laser, 561 nm
HeNe Laser, Orange, 594 nm
White Light Laser 470-670nm, (pulsed, up to 80kHz)
Objectives:
Air:
10x/0.30, HCPL FLUOTAR
20x/0.75 CS2, HC PL APO
Immersion
20x/0.75 IMM CORR CS2, HC PL APO (water, glycerol, oil)
40x/1.10 W CORR CS2, HC PL APO (water)
63x/1.20 W CORR CS2, HC PL APO (water)
Scanner:
Tandem Scanner
Non-resonant mode: 1-1800Hz
Resonant mode: 16000 Hz (bidirectional)
Detectors:
Transmitted light:
1 PMT
Confocal Imaging:
2 PMT
1 HyD
2 HyD SMD (suitable for FLIM and FCS)
Beam Splitter:
AOBS
Software:
LAS X, FALCON, Huygens Lightning Deconvolution
FALCON Time-Correlated Single Photon Counting System:
The SP8 confocal microscope is equipped with 2 HyD SMD detectors and the FALCON time-correlated single photon counting system and corresponding software. This allows two-channel fluorescence lifetime imaging (FLIM) including Förster resonance energy transfer (FRET) measurements as well as fluorescence correlation spectroscopy (FCS).